Calcium phosphate bone cement with 10wt% platelet-rich plasma in vitro and in vivo

Chen, Jian-Chih; Ko, Chia-Ling; Shih, Chi-Jen; Tien, Yin-Chun; Chen, Wen-Cheng

doi:10.1016/j.jdent.2011.11.003

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Volume 40, Issue 2 , Pages 114-122, February 2012

Calcium phosphate bone cement with 10wt% platelet-rich plasma in vitro and in vivo

Jian-Chih Chen
- Department of Orthopaedics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan
- These authors contributed equally to this work.
Chia-Ling Ko
- School of Dentistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Chi-Jen Shih
- Department of Fragrance and Cosmetics Science, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Yin-Chun Tien
- Department of Orthopaedics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Wen-Cheng Chen
- Department of Fiber and Composite Materials, College of Engineering, Feng Chia University, 100 Wenhwa Rd., Seatwen, Taichung 40724, Taiwan, ROC
- These authors contributed equally to this work.
- Corresponding author. Tel.: +886 4 24517250x3413; fax: +886 4 24514625.

Received 8 August 2011; received in revised form 26 October 2011; accepted 1 November 2011. published online 11 November 2011.

Abstract

Objectives

The aim of this study was to evaluate the performance of a 10wt% platelet-rich plasma (PRP) additive composite with calcium phosphate cement (CPC) in vitro and in vivo.

Methods

The in vitro testing of modulus, the apatite conversion rate, morphology, cell and alkaline phosphatase (ALP) activities, and in vivo testing of histological examinations between two groups of 10wt% PRP/CPC and CPC were characterised and compared.

Results

Although the crystallite morphologies showed a retarded effect in the PRP/CPC group in vitro, the modulus results showed that the 10wt% PRP/CPC group had a significant reduction in strength but had no significant changes in the relative conversion ratio of the apatite phase with CPC only. The osteogenic evaluation of ALP expression was significantly increased by the PRP additives group with stem cells (D1) cultured for different periods (2–32days). Our histological examinations showed that greater remodelling and the phenomenon of isolated/detached CPC particles were significantly observed at 9weeks after implantation when the 10wt% PRP/CPC composite was used.