Saliva substitute in combination with high-concentrated fluoride toothpaste: Effects on demineralised dentin in vitro

Tschoppe, Peter; Zandim, Daniela L.; Sampaio, José E.C.; Kielbassa, Andrej M.

doi:10.1016/j.jdent.2009.10.005

« Previous Next »Journal of Dentistry
Volume 38, Issue 3 , Pages 207-213, March 2010

Saliva substitute in combination with high-concentrated fluoride toothpaste: Effects on demineralised dentin in vitro

Peter Tschoppe
- Department of Operative Dentistry and Periodontology, University School of Dental Medicine, CharitéCentrum 3, Charité - Universitätsmedizin Berlin, Germany
- Corresponding author at: Abteilung für Zahnerhaltungskunde und Parodontologie, CharitéCentrum 3 für Zahn-, Mund- und Kieferheilkunde, Charité - Universitätsmedizin Berlin, Assmannshauser Strasse 4-6, 14197 Berlin, Germany. Tel.: +49 030 450 662 327; fax: +49 030 450 562 932.
Daniela L. Zandim
- Department of Operative Dentistry and Periodontology, University School of Dental Medicine, CharitéCentrum 3, Charité - Universitätsmedizin Berlin, Germany
- Department of Oral Diagnosis and Surgery, São Paulo State University, School of Dentistry at Araraquara, São Paulo, Brazil
José E.C. Sampaio
- Department of Oral Diagnosis and Surgery, São Paulo State University, School of Dentistry at Araraquara, São Paulo, Brazil
Andrej M. Kielbassa
- Department of Operative Dentistry and Periodontology, University School of Dental Medicine, CharitéCentrum 3, Charité - Universitätsmedizin Berlin, Germany

Received 24 August 2009; received in revised form 15 October 2009; accepted 19 October 2009. published online 26 October 2009.

Abstract

Objectives

The aim of this in vitro study was to assess the effects of saliva substitutes (modified with respect to calcium, phosphates, and fluorides) in combination with a high-concentrated fluoride toothpaste on demineralised dentin.

Methods

Before and after demineralisation of bovine dentin specimens (subsurface lesions; 37°C, pH 5.0, 5 d), one-quarter of each specimen's surface was covered with nail varnish (control of sound/demineralised tissue). Subsequently, specimens were exposed to original Saliva natura (saturation with respect to octacalciumphosphate [S_OCP]: 0.03; SN 0), or to three lab-produced Saliva natura modifications (S_OCP: 1, 2, and 3; SN 1–3) for 2 and 5 weeks (37°C). An aqueous solution (S_OCP: 2.5) served as positive control (PC). Two times daily (2min each), Duraphat toothpaste (5000ppmF⁻; Colgate)/saliva substitute slurry (ratio 1:3) was applied gently. Differences in mineral losses (ΔΔZ) and lesion depths (ΔLD) between values before and after exposure were microradiographically evaluated.

Results

After both treatment periods specimens immersed in SN 0 revealed significantly higher mineral losses (lower ΔΔZ values) and lesion depths (lower ΔLD) compared to PC (p<0.05; ANOVA). After 5 weeks, specimens stored in SN 1 and 2 showed significantly higher mineral losses compared to PC (p<0.05), while those stored in SN 3 showed similar results (p>0.05). No differences in mineral loss could be observed between SN 2 and 3 (p>0.05).

Conclusions

Under the conditions of this limited protocol, the combination of Saliva natura solutions slightly saturated with respect to OCP in combination with a high-concentrated fluoride toothpaste enabled remineralisation of dentin in vitro.

Keywords: In vitro, Bovine dentin, Fluoridated toothpaste, Hyposalivation, Saliva substitute, Octacalcium phosphate saturation, Demineralisation, Remineralisation, Microradiography