In vitro analysis of inhibitory effects of the antibacterial monomer MDPB-containing restorations on the progression of secondary root caries

Thomé, Thaís; Mayer, Marcia P.A.; Imazato, Satoshi; Geraldo-Martins, Vinícius R.; Marques, Márcia M.

doi:10.1016/j.jdent.2009.05.024

« Previous Next »Journal of Dentistry
Volume 37, Issue 9 , Pages 705-711, September 2009

In vitro analysis of inhibitory effects of the antibacterial monomer MDPB-containing restorations on the progression of secondary root caries

Thaís Thomé
- Departamento de Dentística, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, Brazil
Marcia P.A. Mayer
- Departamento de Microbiologia, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil
Satoshi Imazato
- Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan
Vinícius R. Geraldo-Martins
- Departamento de Dentística, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, Brazil
Márcia M. Marques
- Departamento de Dentística, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, Brazil
- Corresponding author at: Universidade de São Paulo, Faculdade de Odontologia, Departamento de Dentística, Avenida Professor Lineu Prestes, 2227, Cidade Universitária, CEP 05508-900, São Paulo, SP, Brazil. Tel.: +55 11 30917839x213.

Received 10 March 2009; received in revised form 15 May 2009; accepted 19 May 2009. published online 29 May 2009.

Abstract

Objective

This study aimed to analyze in vitro inhibitory effects of restorative materials containing the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on the formation of artificial secondary root caries lesions.

Methods

Class V cavities (2mm×2mm) were prepared in 75 human root fragments. Specimens were randomly divided into five groups (n=15 fragments per group) and restored as follows: (I) MDPB-free adhesive system+MDPB-free composite (negative control); (II) resin modified glass ionomer (RM-GIC; positive control); (III) MDPB-free adhesive system+MDPB-containing composite (2.83% MDPB); (IV) MDPB-containing adhesive system+MDPB-free composite; (V) MDPB-containing adhesive system+MDPB-containing composite. Artificial secondary root caries lesions were produced by a biological artificial caries challenge. The restored specimens were immersed into a culture medium containing Streptococcus mutans and sucrose for 15 days. Histological slices (80±20μm) of the specimens were used for measuring the mean depths of the artificial lesions produced in both margins of the restorations using polarized light microscopy. Results were expressed in percentage related to the mean depth of the negative control, considered 100%. Data were compared by ANOVA followed by the Tukey's test (p≤0.05).

Results

The depths of lesions adjacent to cavities filled with RM-GIC (GII; 85.17±15.2%) were significantly (p<0.01) shallower than those adjacent to restorations with MDPB-free composite (GI; 100.00±10.04%), despite the presence of MDPB in the adhesive system (GIV; 101.95±21.32%). The depths of lesions adjacent to cavities restored with MDPB-containing composite (GIII; 82.68±12.81% and GV; 85.65±15.42%), despite the adhesive system used, were similar to those of RM-GIC (GII). Mean lesions depths in these groups decreased from 13% (GV) to 17% (GIII) in relation to the negative control (GI).