Denture disinfection by microwave irradiation: A randomized clinical study

Ribeiro, Daniela Garcia; Pavarina, Ana Cláudia; Dovigo, Lívia Nordi; Palomari Spolidorio, Denise Madalena; Giampaolo, Eunice Teresinha; Vergani, Carlos Eduardo

doi:10.1016/j.jdent.2009.04.009

« Previous Next »Journal of Dentistry
Volume 37, Issue 9 , Pages 666-672, September 2009

Denture disinfection by microwave irradiation: A randomized clinical study

Daniela Garcia Ribeiro
- R. Humaitá n° 1680, CEP 14801-903, Department of Dental Materials and Prosthodontics, São Paulo State University, UNESP, Araraquara Dental School, Araraquara, São Paulo, Brazil
Ana Cláudia Pavarina
- R. Humaitá n° 1680, CEP 14801-903, Department of Dental Materials and Prosthodontics, São Paulo State University, UNESP, Araraquara Dental School, Araraquara, São Paulo, Brazil
- Corresponding author at: Faculdade de Odontologia de Araraquara - UNESP, R. Humaitá n° 1680, CEP 14801-903, Araraquara, São Paulo, Brazil. Tel.: +55 016 33016410; fax: +55 016 33016406.
Lívia Nordi Dovigo
- R. Humaitá n° 1680, CEP 14801-903, Department of Dental Materials and Prosthodontics, São Paulo State University, UNESP, Araraquara Dental School, Araraquara, São Paulo, Brazil
Denise Madalena Palomari Spolidorio
- R. Humaitá n° 1680, CEP 14801-903, Department of Physiology and Pathology, São Paulo State University, UNESP, Araraquara Dental School, Araraquara, São Paulo, Brazil
Eunice Teresinha Giampaolo
- R. Humaitá n° 1680, CEP 14801-903, Department of Dental Materials and Prosthodontics, São Paulo State University, UNESP, Araraquara Dental School, Araraquara, São Paulo, Brazil
Carlos Eduardo Vergani
- R. Humaitá n° 1680, CEP 14801-903, Department of Dental Materials and Prosthodontics, São Paulo State University, UNESP, Araraquara Dental School, Araraquara, São Paulo, Brazil

Received 22 January 2009; received in revised form 16 April 2009; accepted 24 April 2009. published online 05 May 2009.

Abstract

Objective

This study evaluated the clinical effectiveness of two exposure times of microwave irradiation on the disinfection of complete dentures.

Methods

Biofilm samples were collected from dentures of 30 patients, who were randomly divided into two experimental groups of 15 subjects each: Group 1—patients had their maxillary denture microwaved for 3min (650W); Group 2—patients had their maxillary denture microwaved for 2min (650W). Denture biofilm samples were taken with swabs, before (left side surfaces) and after (right side surfaces) microwave irradiation. All microbial material was plated on selective media for Candida spp., Staphylococcus spp., mutans streptococci and a non-selective media. After incubation (48h/37°C), the number of colony-forming units (cfu/mL) was counted. Microorganisms which grew on selective media were identified using biochemical methods. The data were statistically analyzed by Kruskal–Wallis test, followed by Dunn's post-test (α=0.05).

Results

Microwave irradiation for 3min (Group 1) resulted in sterilization of all dentures evaluated. After microwave irradiation for 2min (Group 2), a significant decrease in Candida spp. (P=0.0062), Staphylococcus spp. (P=0.0178), mutans streptococci (P=0.0047) and non-identified species (P<0.0001) was achieved in comparison with the cfu/mL obtained before irradiation. The colonies grown after 2min of microwave irradiation were identified as Candida albicans, non-aureus Staphylococci and Streptococcus mutans.