Root dentin body moisture changes caused by ascorbic acid

Komabayashi, Takashi; Ahn, Chul; Zhang, Song; Zhu, Qiang; Spångberg, Larz S.W.

doi:10.1016/j.jdent.2009.03.004

« Previous Next »Journal of Dentistry
Volume 37, Issue 6 , Pages 475-479, June 2009

Root dentin body moisture changes caused by ascorbic acid

Takashi Komabayashi
- Department of Endodontics, Texas A&M Health Science Center Baylor College of Dentistry, 3302 Gaston Avenue, Dallas, TX 75246, USA
- Corresponding author. Tel.: +1 214 828 8365; fax: +1 214 874 4507.
Chul Ahn
- Department of Clinical Sciences, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390-9066, USA
Song Zhang
- Department of Clinical Sciences, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390-9066, USA
Qiang Zhu
- University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
Larz S.W. Spångberg
- University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA

Received 12 January 2009; received in revised form 8 March 2009; accepted 10 March 2009.

Abstract

Objectives

To quantify in vitro the root dentin moisture (DM) when soaked in 10% ascorbic acid solution (Group A) and distilled water (Group B) for up to 14 days.

Methods

Forty-four extracted human mandibular single-rooted teeth were sectioned perpendicular to the long axis at the CEJ to access the root dentin. The samples were divided into Groups A and B. Baseline (day 0) DM was measured using a digital grain moisture meter. One sample was placed in each tube, which was then filled with solution. All tubes were kept at 37°C and 100% humidity. DM was measured after 1, 3, 7, and 14 days. The baseline DM value was used as a covariate in the generalized estimating equation (GEE) analysis to account for the difference in the baseline DM between the two groups.

Results

The mean DM (%) values±standard deviation on days 0, 1, 3, 7, and 14 were 11.4±1.08, 17.1±0.87, 18.2±0.76, 18.4±0.77, and 17.9±0.90 in Group A, and 10.2±0.95, 12.8±0.90, 13.3±0.95, 13.0±0.91, and 13.2±0.89 in Group B, respectively. Group A had significantly higher baseline DM than Group B (p=0.006). After adjusting for differences in the baseline, the GEE analysis indicated that, on average, Group A had a significantly higher increase in DM than Group B, with means±standard deviation of 4.35±0.26.

Conclusions

The moisture increase in the ascorbic acid group was greater than that in the distilled water group. Soaking root dentin in the unreplenished ascorbic acid solution or distilled water beyond 3 days did not further increase DM.

Keywords: Ascorbic acid, Bonding, Dentin moisture, Generalized estimating equation analysis, Rapid non-destructive method