Journal of Dentistry
Volume 37, Issue 7 , Pages 502-508 , July 2009

Efficient gene transfer to periodontal ligament cells and human gingival fibroblasts by adeno-associated virus vectors

  • Melanie Kunze

      Affiliations

    • Center of Dental Medicine, Department of Operative Dentistry and Periodontology, University of Cologne, Germany
    • ,
  • Anke Huber

      Affiliations

    • Clinic I for Internal Medicine, University of Cologne, Kerpener Str. 62, 50937 Cologne, Germany
    • ,
  • Anna Krajewski

      Affiliations

    • Center of Dental Medicine, Department of Operative Dentistry and Periodontology, University of Cologne, Germany
    • ,
  • Esther Lowden

      Affiliations

    • Center of Dental Medicine, Department of Operative Dentistry and Periodontology, University of Cologne, Germany
    • ,
  • Natascha Schuhmann

      Affiliations

    • Clinic I for Internal Medicine, University of Cologne, Kerpener Str. 62, 50937 Cologne, Germany
    • ,
  • Hildegard Buening

      Affiliations

    • Clinic I for Internal Medicine, University of Cologne, Kerpener Str. 62, 50937 Cologne, Germany
    • Center for Molecular Medicine, University of Cologne, Germany
    • ,
  • Michael Hallek

      Affiliations

    • Clinic I for Internal Medicine, University of Cologne, Kerpener Str. 62, 50937 Cologne, Germany
    • Center for Molecular Medicine, University of Cologne, Germany
    • ,
  • Michael Noack

      Affiliations

    • Center of Dental Medicine, Department of Operative Dentistry and Periodontology, University of Cologne, Germany
    • ,
  • Luca Perabo

      Affiliations

    • Clinic I for Internal Medicine, University of Cologne, Kerpener Str. 62, 50937 Cologne, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49 221 478 4469; fax: +49 221 478 87941.

Received 17 January 2009 ,Accepted 8 March 2009.

References 

  1. Baum BJ, O’Connell BC. The impact of gene therapy on dentistry. Journal of the American Dental Association. 1995;126:179–189
  2. Verma IM, Weitzman MD. Gene therapy: twenty-first century medicine. Annual Review of Biochemistry. 2005;74:711–738
  3. Anusaksathien O, Webb SA, Jin QM, Giannobile WV. Platelet-derived growth factor gene delivery stimulates ex vivo gingival repair. Tissue Engineering. 2003;9:745–756
  4. Anusaksathien O, Jin Q, Zhao M, Somerman MJ, Giannobile WV. Effect of sustained gene delivery of platelet-derived growth factor or its antagonist (PDGF-1308) on tissue-engineered cementum. Journal of Periodontology. 2004;75:429–440
  5. Chen QP, Giannobile WV. Adenoviral gene transfer of PDGF downregulates gas gene product PDGFalphaR and prolongs ERK and Akt/PKB activation. American Journal of Physiology: Cell Physiology. 2002;282:C538–C544
  6. Zhu Z, Lee CS, Tejeda KM, Giannobile WV. Gene transfer and expression of platelet-derived growth factors modulate periodontal cellular activity. Journal of Dental Research. 2001;80:892–897
  7. Giannobile WV, Lee CS, Tomala MP, Tejeda KM, Zhu Z. Platelet-derived growth factor (PDGF) gene delivery for application in periodontal tissue engineering. Journal of Periodontology. 2001;72:815–823
  8. Jin Q, Anusaksathien O, Webb SA, Printz MA, Giannobile WV. Engineering of tooth-supporting structures by delivery of PDGF gene therapy vectors. Molecular Therapy. 2004;9:519–526
  9. Jin QM, Anusaksathien O, Webb SA, Rutherford RB, Giannobile WV. Gene therapy of bone morphogenetic protein for periodontal tissue engineering. Journal of Periodontology. 2003;74:202–213
  10. Dunn CA, Jin Q, Taba M, Franceschi RT, Bruce Rutherford R, Giannobile WV. BMP gene delivery for alveolar bone engineering at dental implant defects. Molecular Therapy. 2005;11:294–299
  11. Kanzaki H, Chiba M, Takahashi I, Haruyama N, Nishimura M, Mitani H. Local OPG gene transfer to periodontal tissue inhibits orthodontic tooth movement. Journal of Dental Research. 2004;83:920–925
  12. Chen R, Kanzaki H, Chiba M, Nishimura M, Kanzaki R, Igarashi K. Local osteoprotegerin gene transfer to periodontal tissue inhibits lipopolysaccharide-induced alveolar bone resorption. Journal of Periodontal Research. 2008;43:237–245
  13. Wu Z, Asokan A, Samulski RJ. Adeno-associated virus serotypes: vector toolkit for human gene therapy. Molecular Therapy. 2006;14:316–327
  14. Grimm D, Kay MA. From virus evolution to vector revolution: use of naturally occurring serotypes of adeno-associated virus (AAV) as novel vectors for human gene therapy. Current Gene Therapy. 2003;3:281–304
  15. Le Bec C, Douar AM. Gene therapy progress and prospects—vectorology: design and production of expression cassettes in AAV vectors. Gene Therapy. 2006;13:805–813
  16. Buning H, Perabo L, Coutelle O, Quadt-Humme S, Hallek M. Recent developments in adeno-associated virus vector technology. Journal of Gene Medicine. 2008;10:717–733
  17. Grieger JC, Choi VW, Samulski RJ. Production and characterization of adeno-associated viral vectors. Nature Protocols. 2006;1:1412–1428
  18. Monahan PE, Jooss K, Sands MS. Safety of adeno-associated virus gene therapy vectors: a current evaluation. Expert Opinion on Drug Safety. 2002;1:79–91
  19. Coura Rdos S, Nardi NB. The state of the art of adeno-associated virus-based vectors in gene therapy. Virology Journal. 2007;4:99
  20. Mueller C, Flotte TR. Clinical gene therapy using recombinant adeno-associated virus vectors. Gene Therapy. 2008;15:858–863
  21. Jennings K, Miyamae T, Traister R, Marinov A, Katakura S, Sowders D, et al. Proteasome inhibition enhances AAV-mediated transgene expression in human synoviocytes in vitro and in vivo. Molecular Therapy. 2005;11:600–607
  22. Katakura S, Jennings K, Watanabe S, Adachi E, Thornton S, Gao GP, et al. Recombinant adeno-associated virus preferentially transduces human, compared to mouse, synovium: implications for arthritis therapy. Moder Rheumatology. 2004;14:18–24
  23. Boissier MC, Lemeiter D, Clavel C, Valvason C, Laroche L, Beque T, et al. Synoviocyte infection with adeno-associated virus (AAV) is neutralized by human synovial fluid from arthritis patients and depends on AAV serotype. Human Gene Therapy. 2007;18:525–535
  24. Adriaansen J, Vervoordeldonk MJ, Tak PP. Gene therapy as a therapeutic approach for the treatment of rheumatoid arthritis: innovative vectors and therapeutic genes. Rheumatology (Oxford). 2006;45:656–668
  25. McCarty DM. Self-complementary AAV vectors; advances and applications. Molecular Therapy. 2008;16:1648–1656
  26. Xiao X, Li J, Samulski RJ. Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus. Journal of Virology. 1998;72:2224–2232
  27. Rabinowitz JE, Rolling F, Li C, Conrath H, Xiao W, Xiao X, et al. Cross-packaging of a single adeno-associated virus (AAV) type 2 vector genome into multiple AAV serotypes enables transduction with broad specificity. Journal of Virology. 2002;76:791–801
  28. Hacker UT, Wingenfeld L, Kofler DM, Schuhmann NK, Lutz S, Herold T, et al. Adeno-associated virus serotypes 1 to 5 mediated tumor cell directed gene transfer and improvement of transduction efficiency. Journal of Gene Medicine. 2005;7:1429–1438
  29. Ramseier CA, Abramson ZR, Jin Q, Giannobile WV. Gene therapeutics for periodontal regenerative medicine. Dental Clinics of North America. 2006;50:245–263
  30. Karthikeyan BV, Pradeep AR. Gene therapy in periodontics: a review and future implications. Journal of Contemporary Dental Practice. 2006;7:83–91
  31. Girod A, Ried M, Wobus C, Lahm H, Leike K, Kleinschmidt J, et al. Genetic capsid modifications allow efficient re-targeting of adeno-associated virus type 2. Nature Medicine. 1999;5:1438
  32. Perabo L, Buning H, Kofler DM, Ried M, Girod A, Wendtner CM, et al. In vitro selection of viral vectors with modified tropism: the adeno-associated virus display. Molecular Therapy. 2003;8:151–157
  33. Muller OJ, Kaul F, Weitzman MD, Pasqualini R, Arap W, Kleinschmidt JA, et al. Random peptide libraries displayed on adeno-associated virus to select for targeted gene therapy vectors. Nature Biotechnology. 2003;21:1040–1046
  34. Ghosh A, Yue Y, Lai Y, Duan D. A hybrid vector system expands adeno-associated viral vector packaging capacity in a transgene-independent manner. Molecular Therapy. 2008;16:124–130
  35. Perabo L, Endell J, King S, Lux K, Goldnau D, Hallek M, et al. Combinatorial engineering of a gene therapy vector: directed evolution of adeno-associated virus. Journal of Gene Medicine. 2006;8:155–162
  36. Maheshri N, Koerber JT, Kaspar BK, Schaffer DV. Directed evolution of adeno-associated virus yields enhanced gene delivery vectors. Nature Biotechnology. 2006;24:198–204
  37. Erles K, Sebokova P, Schlehofer JR. Update on the prevalence of serum antibodies (IgG and IgM) to adeno-associated virus (AAV). Journal of Medical Virology. 1999;59:406–411
  38. Chirmule N, Propert K, Magosin S, Qian Y, Qian R, Wilson J. Immune responses to adenovirus and adeno-associated virus in humans. Gene Therapy. 1999;6:1574–1583
  39. Moskalenko M, Chen L, van Roey M, Donahue BA, Snyder RO, McArthur JG, et al. Epitope mapping of human anti-adeno-associated virus type 2 neutralizing antibodies: implications for gene therapy and virus structure. Journal of Virology. 2000;74:1761–1766
  40. Hauck B, Zhao W, High K, Xiao W. Intracellular viral processing, not single-stranded DNA accumulation, is crucial for recombinant adeno-associated virus transduction. Journal of Virology. 2004;78:13678–13686

☆ This work was supported by grants of the German Research Foundation (DFG) and Köln Fortune Program.

PII: S0300-5712(09)00047-5

doi: 10.1016/j.jdent.2009.03.001

Journal of Dentistry
Volume 37, Issue 7 , Pages 502-508 , July 2009

Article Tools

* Image Usage & Resolution