In vitro evaluation of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) effect on stripped enamel surfaces. A SEM investigation

Giulio, Alessandri Bonetti; Matteo, Zanarini; Serena, Incerti Parenti; Silvia, Marchionni; Luigi, Checchi

doi:10.1016/j.jdent.2008.11.015

« Previous Next »Journal of Dentistry
Volume 37, Issue 3 , Pages 228-232, March 2009

In vitro evaluation of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) effect on stripped enamel surfaces. A SEM investigation

Alessandri Bonetti Giulio
- Department of Orthodontics, Alma Mater Studiorum University of Bologna, Bologna, Italy
- Corresponding author at: Department of Orthodontics, Alma Mater Studiorum, University of Bologna, Via San Vitale 59, 40125 Bologna, Italy. Tel.: +39 0512088133; fax: +39 051225208.
Zanarini Matteo
- Department of Orthodontics, Alma Mater Studiorum University of Bologna, Bologna, Italy
Incerti Parenti Serena
- Department of Orthodontics, Alma Mater Studiorum University of Bologna, Bologna, Italy
Marchionni Silvia
- Electron Microscopy Laboratory, Department of Dental Sciences, Alma Mater Studiorum, University of Bologna, Bologna, Italy
Checchi Luigi
- Department of Periodontology, Alma Mater Studiorum University of Bologna, Bologna, Italy

Received 15 September 2008; received in revised form 6 November 2008; accepted 12 November 2008.

Summary

Objectives

Interproximal enamel reduction (orthodontic stripping) leaves surface-roughness that encourages plaque adherence. Sealants and fluoride products have been recommended to avoid possible detrimental effects. The objective of this study was to qualitatively evaluate, by means of scanning electron microscopy (SEM), the effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on stripped enamel morphology after exposure to an acid solution.

Methods

15 extracted lower incisors underwent stripping with metal strips (Horico, 80μm) by 1 operator. Mesial and distal surfaces were sectioned into 30 samples (22 stripped; 8 unstripped) and assigned to 2 groups: Group A (2 stripped; 2 unstripped) served as control; in Group B (20 stripped; 6 unstripped) tooth mousse containing CPP-ACP was tested. For 8 days, teeth were placed in acid lactic solution for 2h three times a day (from 9a.m. to 9p.m.) with 2h water preservation in between. After each demineralizing bath, samples of group B were coated with CPP-ACP for 5min before immersion into water.

Results

In Group A, stripped samples exhibited greater demineralization compared to unstripped. In Group B, CPP-ACP reduced enamel dissolution on both intact and abraded samples compared to Group A.

Conclusions

Injuries to enamel morphology caused by stripping might predispose to greater demineralization in the event of exposure to acid environment. Topical applications of CPP-ACP could be effective in promoting enamel remineralization after interdental stripping.

Keywords: Interproximal enamel reduction, Stripping, Demineralization, Remineralization, CPP-ACP