The influence of 30-day-old Streptococcus mutans biofilm on the surface of esthetic restorative materials—An in vitro study

Fúcio, Suzana B.P.; Carvalho, Fabíola G.; Sobrinho, Lourenço Correr; Sinhoreti, Mário A.C.; Puppin-Rontani, Regina M.

doi:10.1016/j.jdent.2008.06.002

« Previous Next »Journal of Dentistry
Volume 36, Issue 10 , Pages 833-839, October 2008

The influence of 30-day-old Streptococcus mutans biofilm on the surface of esthetic restorative materials—An in vitro study

Suzana B.P. Fúcio
- Department of Restorative Dentistry, Dental Material Area, Faculty of Dentistry of Piracicaba, State University of Campinas, Postal Code 52, São Paulo Brazil
Fabíola G. Carvalho
- Department of Restorative Dentistry, Dental Material Area, Faculty of Dentistry of Piracicaba, State University of Campinas, Postal Code 52, São Paulo Brazil
Lourenço Correr Sobrinho
- Department of Restorative Dentistry, Dental Material Area, Faculty of Dentistry of Piracicaba, State University of Campinas, Postal Code 52, São Paulo Brazil
Mário A.C. Sinhoreti
- Department of Restorative Dentistry, Dental Material Area, Faculty of Dentistry of Piracicaba, State University of Campinas, Postal Code 52, São Paulo Brazil
Regina M. Puppin-Rontani
- Department of Pediatric Dentistry, Faculty of Dentistry of Piracicaba, State University of Campinas, Postal Code 52, São Paulo, Brazil
- Corresponding author at: Pediatric Dentistry Area, Department of Pediatric Dentistry, Piracicaba Dental School, UNICAMP, Av. Limeira 901, 13414-900 Piracicaba, SP, Brazil. Tel.: +55 19 2106 5286; fax: +55 19 2106 5218.

Received 5 March 2008; received in revised form 5 June 2008; accepted 5 June 2008.

Abstract

Objective

To evaluate the effects of Streptococcus mutans biofilm/restorative materials interaction on surface roughness, hardness and morphology of materials tested.

Methods

Empress 2 (E2), Filtek Supreme (FS), Vitremer (V) and Ketac Molar Easymix (KM) were tested. Twenty-five disks of each material were made and divided into three storage groups: (1) 100% relative humidity (n=5); (2) growth medium (BHI and 1% sucrose) (n=5); (3) S. mutans biofilm-growth medium (n=15). Before storage, hardness measurements were immediately obtained from group 1 specimens. After 30 days of storage, the specimens were cleaned in order to obtain the surface roughness and hardness values, besides morphology analysis by scanning electron microscopy.

Results

The surface roughness and hardness values obtained from E2 and FS specimens did not present statistically significant differences among the groups 1, 2 and 3 and between immediate and 30-day-old specimens of each material. However, group 3 specimens of V and KM showed statistically significant higher surface roughness means than other groups. Group 1 specimens of V and KM also showed higher hardness values than the immediate values. Group 3 specimens of V presented decreased hardness values compared with other groups. The scanning electron micrographs showed an increase in surface degradation from group 1 to group 3 for FS, V and KM.

Conclusions

Thirty-day-old biofilm promotes a negative effect on the surface morphology of FS, V and KM, on the surface roughness of V and KM and on the hardness of V.

Keywords: Streptococcus mutans, Biodegradation, Dental plaque, Materials analysis, Hardness, Ultrastructure, Composite resin, Dental porcelain, Glass ionomer cements