Influence of human dentine on the antibacterial activity of self-etching adhesive systems against cariogenic bacteria

Gondim, Juliana O.; Duque, Cristiane; Hebling, Josimeri; Giro, Elisa M.A.

doi:10.1016/j.jdent.2007.12.007

« Previous Next »Journal of Dentistry
Volume 36, Issue 4 , Pages 241-248, April 2008

Influence of human dentine on the antibacterial activity of self-etching adhesive systems against cariogenic bacteria

Juliana O. Gondim
- Department of Pediatric Dentistry, School of Dentistry of Araraquara, São Paulo State University, UNESP, Rua Humaitá, 1680 Araraquara, SP, Brazil
Cristiane Duque
- Department of Oral Diagnosis, School of Dentistry of Piracicaba, São Paulo State University, UNICAMP, Av. Limeira, 907 Piracicaba, SP, Brazil
Josimeri Hebling
- Department of Pediatric Dentistry, School of Dentistry of Araraquara, São Paulo State University, UNESP, Rua Humaitá, 1680 Araraquara, SP, Brazil
Elisa M.A. Giro
- Department of Pediatric Dentistry, School of Dentistry of Araraquara, São Paulo State University, UNESP, Rua Humaitá, 1680 Araraquara, SP, Brazil
- Corresponding author at: Faculdade de Odontologia de Araraquara, Departamento de Clínica Infantil Rua Humaitá, 1680 Centro Araraquara, SP, CEP 14801-903, Brazil. Tel.: +55 16 3301 6336.

Received 27 September 2007; received in revised form 7 December 2007; accepted 22 December 2007.

Abstract

Objectives

The incorporation of antibacterial agents into adhesive systems has been proposed to eliminate residual bacteria from dentine. This study used the agar diffusion method to evaluate the antibacterial activity of Clearfil Protect Bond (CPB), Clearfil SE Bond (CSEB), Clearfil Tri-S Bond (C3SB) and Xeno-III (XIII) self-etching adhesive systems, with or without light-activation, against cariogenic bacteria, and to assess the influence of human dentine on the antibacterial activity of these materials.

Methods

An aliquot of 10μl per material (and individual components) were pipetted onto paper and dentine discs distributed in Petri dishes containing bacterial culture in BHI agar. Positive control was 0.2% chlorhexidine digluconate (CHX).

Results

After incubation, the adhesive components of CPB and CSEB, liquid A of XIII and C3SB did not present antibacterial activity when applied to paper discs. The non-light-activated CPB primer+adhesive promoted the greatest inhibition of Streptococcus mutans (p<0.05), whereas with light-activation, there was no significant difference between primer+adhesive and primer alone. For Lactobacillus acidophilus, CPB primer presented the greatest antibacterial activity in both light-activation conditions (p<0.05). Regarding the dentine discs, only CHX promoted an inhibitory effect, though less intense than on paper discs (p<0.05). CHX presented greater antibacterial activity against S. mutans than against L. acidophilus (p<0.05).

Conclusions

Light-activation significantly reduced the antibacterial activity of the self-etching adhesive systems; MDPB incorporation contributed to the effect of adhesive systems against cariogenic bacteria; the components eluted from the adhesive systems were not capable to diffuse through 400μm-thick dentine disc to exert their antibacterial activity against cariogenic bacteria.

Keywords: Dentine bonding agents, Bacteria, Antibacterial agents, Dentine, Dentine permeability