Journal of Dentistry
Volume 35, Issue 10 , Pages 794-799, October 2007

Enamel diffusion modulated by Er:YAG laser:

(Part 2). Organic matrix

  • N.L. Maung

      Affiliations

    • Department of Preventive Dentistry, Faculty of Dentistry, 5 Lower Kent Ridge Road, National University of Singapore, Singapore 119074
    • ,
  • T. Wohland

      Affiliations

    • Department of Chemistry, 3 Science Drive 3, National University of Singapore, Singapore 117543
    • ,
  • C.-Y.S. Hsu

      Affiliations

    • Department of Preventive Dentistry, Faculty of Dentistry, 5 Lower Kent Ridge Road, National University of Singapore, Singapore 119074
    • Corresponding Author InformationCorresponding author. Tel.: +65 6779 5555x1658; fax: +65 6773 2602.

Received 29 March 2007; received in revised form 8 July 2007; accepted 16 July 2007.

Summary 

Organic matrix (OM) has been hypothesized as a key player in the laser-induced retardation of enamel diffusion (LRED).

Objectives

Therefore, this study was aimed to quantify the contribution of OM in LRED.

Methods

Four groups of enamel sections (n=10) were assigned to ‘normal’, ‘laser treated’, ‘OM extracted’ and ‘laser+OM extraction’ groups for measurement of diffusion coefficient (DC) using fluorescence recovery after photobleaching (FRAP) and fluorophores transport study (FTS). Er:YAG laser treatment and OM extraction were performed on respective groups. Sections were characterized with stereomicroscopy and polarized light microscopy. Treatment effects were statistically assessed with a factorial ANOVA.

Results

DC measured by FRAP and FTS coupled with confocal microscopy revealed the significant effect of OM (p=0.001) and laser treatment (p<0.01). After OM extraction, the laser effect on diffusion decreased about 34–75%, confirming the significant role of OM in LRED.

Conclusion

Both FRAP and FTS may be promising tools to quantify enamel DC.

Keywords: Enamel, Diffusion, Organic matrix, Er:YAG laser, CLSM

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PII: S0300-5712(07)00138-8

doi:10.1016/j.jdent.2007.07.012

Refers to article:

  • Enamel diffusion modulated by Er:YAG laser: (Part 1)—FRAP

    N.L. Maung, T. Wohland, C.-Y.S. Hsu
    Journal of Dentistry October 2007 (Vol. 35, Issue 10, Pages 787-793)

Journal of Dentistry
Volume 35, Issue 10 , Pages 794-799, October 2007

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