Journal of Dentistry
Volume 35, Issue 8 , Pages 664-672, August 2007

Effect of dentin depth on hybridization quality using different bonding tactics in vivo

  • Y. Yuan

      Affiliations

    • Department of Cariology and Operative Dentistry, Tokyo Medical and Dental University (TMDU), Tokyo, Japan
    • 21st Century Center of Excellence (COE) Program, FRMDRTB at TMDU, Tokyo, Japan
    • Corresponding Author InformationCorresponding author at: 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. Tel.: +81 3 5803 5483; fax: +81 3 5803 0195.
    • ,
  • Y. Shimada

      Affiliations

    • Department of Cariology and Operative Dentistry, Tokyo Medical and Dental University (TMDU), Tokyo, Japan
    • ,
  • S. Ichinose

      Affiliations

    • Instrumental Analysis Research Center, Tokyo Medical and Dental University (TMDU), Tokyo, Japan
    • ,
  • J. Tagami

      Affiliations

    • Department of Cariology and Operative Dentistry, Tokyo Medical and Dental University (TMDU), Tokyo, Japan
    • 21st Century Center of Excellence (COE) Program, FRMDRTB at TMDU, Tokyo, Japan

Received 20 February 2007; received in revised form 2 May 2007; accepted 3 May 2007.

Abstract 

Objectives

Incomplete resin infiltration and polymerization of adhesive contributed to nanoleakage formation. This study tested the null hypothesis that adoption of different bonding tactics and dentine depth will not affect hybridization quality in vivo.

Methods

Class V cavities were prepared on the labial/buccal surface of monkey teeth. They were bonded by Single Bond (a two-step total-etch adhesive), Clearfil SE Bond (a two-step self-etch adhesive), or Clearfil S3 Bond (an all-in-one self-etch adhesive). Combined nanoleakage analysis and quantitative immunolabeling evaluation were carried out in the hybrid layer formed in both cervical superficial and deep dentine.

Results

Single Bond showed reticular and spotted nanoleakage while Clearfil SE Bond and Clearfil S3 Bond presented only a spotted one. While Single Bond showed increased concentration of labeling of type I collagen within the deep part of the hybrid layer, two self-etch adhesives—Clearfil SE Bond and Clearfil S3 Bond revealed a homogeneous labeling pattern, even if the latter presented a significantly increased labeling index in deep dentine.

Conclusions

Different bonding tactics showed different nanoleakage patterns and immunolabeling index, and was influenced by dentine depth at different levels in vivo.

Keywords: Dentin depth, Nanoleakage, TEM, Monoclonal type I collagen antibody, FE-SEM, YAG backscattered electron

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PII: S0300-5712(07)00094-2

doi:10.1016/j.jdent.2007.05.002

Journal of Dentistry
Volume 35, Issue 8 , Pages 664-672, August 2007

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