Journal of Dentistry
Volume 35, Issue 8 , Pages 636-642, August 2007

Dissolution of bio-active dentine matrix components by mineral trioxide aggregate

  • Phillip L. Tomson

      Affiliations

    • Oral Biology, School of Dentistry, The University of Birmingham, St Chad's Queensway, Birmingham B4 6NN, UK
    • Corresponding Author InformationCorresponding author. Tel.: +44 121 237 2877; fax: +44 121 625 8815.
    • ,
  • Liam M. Grover

      Affiliations

    • School of Chemical Engineering, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    • ,
  • Philip J. Lumley

      Affiliations

    • Oral Biology, School of Dentistry, The University of Birmingham, St Chad's Queensway, Birmingham B4 6NN, UK
    • ,
  • Alastair J. Sloan

      Affiliations

    • Department of Oral Surgery, Medicine & Pathology, Cardiff University, Wales College of Medicine, School of Dentistry, Heath Park, Cardiff CF14 4XY, UK
    • ,
  • Anthony J. Smith

      Affiliations

    • Oral Biology, School of Dentistry, The University of Birmingham, St Chad's Queensway, Birmingham B4 6NN, UK
    • ,
  • Paul R. Cooper

      Affiliations

    • Oral Biology, School of Dentistry, The University of Birmingham, St Chad's Queensway, Birmingham B4 6NN, UK

Received 20 February 2007; received in revised form 19 April 2007; accepted 20 April 2007.

Abstract 

Objectives

To analyze the soluble components of setting and set mineral trioxide aggregate (MTA), assess the abilities of two varieties of MTA and Ca(OH)2 solutions to solubilise dentine matrix proteins (DMPs) and determine if these extracts contain signalling molecules important to pulpal repair and regeneration.

Methods

The metallic ion composition of solutions of white and grey MTA (pH 11.7), 0.02M Ca(OH)2 (pH 11.9) and 10% EDTA (pH 7.2) was determined using atomic absorption spectroscopy. Extracellular dentine matrix components from powdered human dentine were extracted using all solutions over 14 days. Extracts were analysed for concentrations of non-collagenous proteins (NCPs) and glycosaminoglycans (GAGs), and protein profiles were examined using 1D-polyacrylamide gel electrophoresis (1D-PAGE). ELISAs for TGF-β1 and adrenomedullin (ADM) were also performed.

Results

Aluminium, calcium, potassium, and sodium ions were detected in both white and grey MTA solutions. MTA and Ca(OH)2 solutions liberated similar amounts of GAGs and NCPs although yields were considerably lower than those obtained using the EDTA solution. 1D-PAGE analysis demonstrated differences in protein profiles solubilised from dentine for all solutions. All extracts contained TGF-β1 and ADM, EDTA solution liberated significantly greater amounts of TGF-β1, and Ca(OH)2 and grey MTA solutions released more ADM.

Conclusions

These data imply that when placed clinically soluble components of set and setting MTA may release dentine matrix components that potentially influence cellular events for dentine repair and regeneration.

Keywords: Mineral trioxide aggregate (MTA), Dentine, Pulp, Regeneration, Growth factors, TGF, ADM

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PII: S0300-5712(07)00077-2

doi:10.1016/j.jdent.2007.04.008

Journal of Dentistry
Volume 35, Issue 8 , Pages 636-642, August 2007

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