Journal of Dentistry
Volume 35, Issue 5 , Pages 377-382, May 2007

Incipient caries lesions on cementum by mono- and co-culture oral bacteria

  • H.K. Yip

      Affiliations

    • Family Dentistry and Endodontics, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong, China
    • Corresponding Author InformationCorresponding author at: Room 1A23, Prince Philip Dental Hosptial, 34 Hospital Road, Hong Kong, China. Tel.: +852 2859 0286; fax: +852 2547 0164.
    • ,
  • J.H. Guo

      Affiliations

    • Key Laboratory of Oral Biomedical Engineering, School & Hospital of Stomatology, Wuhan University, Wuhan, China
    • ,
  • W.H.S. Wong

      Affiliations

    • Division of Biostatistics, Department of Pediatric and Adolescent Medicine, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China

Received 18 August 2006; received in revised form 27 October 2006; accepted 9 November 2006.

Abstract 

Objectives

There is increasing prevalence of root caries. We hypothesized different biofilms will cause varying demineralization in cementum. This study investigated the extent of demineralization of cementum by oral biofilm formed from three major cariogenic microorganisms: Streptococcus mutans, Lactobacillus acidophilus, and Actinomyces israelii. Sound cementum tooth blocks were incubated with mono-, bi-, and tri-species combinations of the bacteria under investigation.

Materials and methods

The matrix (amide I) and phosphate content of the lesions was analyzed by Fourier-transform infrared spectroscopy (FTIR), and calcium and phosphorus levels were analyzed by scanning electron microscopy and energy-dispersive spectroscopy (SEM-EDX).

Results

The log[amide I:HPO42− absorbance] values showed that A. israelii mono-culture caused significantly more demineralization than the other bacterial cultures. log[Ca:P] showed that all carious lesions were confined to the cementum.

Conclusions

Oral biofilm arising from bacterial species A. israelii alone was the most cariogenic of those tested and produced the most demineralization in incipient carious lesions in cementum.

Keywords: Biofilm, Cementum, Demineralization, Incipient lesion, Root surface

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PII: S0300-5712(06)00206-5

doi:10.1016/j.jdent.2006.11.002

Journal of Dentistry
Volume 35, Issue 5 , Pages 377-382, May 2007

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