Journal of Dentistry
Volume 35, Issue 3 , Pages 187-194 , March 2007

Alterations of energy metabolism and glutathione levels of HL-60 cells induced by methacrylates present in composite resins

  • G. Nocca

      Affiliations

    • Biochemistry and Clinical Biochemistry Institute, School of Medicine, Catholic University, Rome, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39 06 3057612; fax: +39 06 3053598.
    • ,
  • F. De Palma

      Affiliations

    • Biochemistry and Clinical Biochemistry Institute, School of Medicine, Catholic University, Rome, Italy
    • ,
  • A. Minucci

      Affiliations

    • Biochemistry and Clinical Biochemistry Institute, School of Medicine, Catholic University, Rome, Italy
    • ,
  • P. De Sole

      Affiliations

    • Biochemistry and Clinical Biochemistry Institute, School of Medicine, Catholic University, Rome, Italy
    • ,
  • G.E. Martorana

      Affiliations

    • Biochemistry and Clinical Biochemistry Institute, School of Medicine, Catholic University, Rome, Italy
    • ,
  • C. Callà

      Affiliations

    • Biochemistry and Clinical Biochemistry Institute, School of Medicine, Catholic University, Rome, Italy
    • ,
  • C. Morlacchi

      Affiliations

    • Biochemistry and Clinical Biochemistry Institute, School of Medicine, Catholic University, Rome, Italy
    • ,
  • M.L. Gozzo

      Affiliations

    • Biochemistry and Clinical Biochemistry Institute, School of Medicine, Catholic University, Rome, Italy
    • ,
  • G. Gambarini

      Affiliations

    • Department of Endodontics, School of Medicine, La Sapienza University, Rome, Italy
    • ,
  • C. Chimenti

      Affiliations

    • Surgical Sciences Department, School of Medicine, University of L’Aquila, L’Aquila, Italy
    • ,
  • B. Giardina

      Affiliations

    • Biochemistry and Clinical Biochemistry Institute, School of Medicine, Catholic University, Rome, Italy
    • Molecular Recognition Chemistry Institute, CNR, Rome, Italy
    • ,
  • A. Lupi

      Affiliations

    • Molecular Recognition Chemistry Institute, CNR, Rome, Italy

Received 23 December 2005 ,Revised 19 July 2006 ,Accepted 25 July 2006.

References 

  1. Peutzfeldt A. Resin composites in dentistry: the monomer systems. European Journal of Oral Science. 1997;105:97–116
  2. Bouillaguet S, Wataha JC, Hanks CT, Ciucchi B, Holz J. In vitro cytotoxicity and dentin permeability of HEMA. Journal of Endodontics. 1996;22:244–248
  3. Bouillaguet S, Virgillito M, Wataha J, Ciucchi B, Holz J. The influence of dentine permeability on cytotoxicity of four dentine bonding systems, in vitro. Journal of Oral Rehabilitation. 1998;25:45–51
  4. Gerzina TM, Hume WR. Diffusion of monomers from bonding resin–resin composite combinations through dentine in vitro. Journal of Dentistry. 1996;24:125–128
  5. Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. Journal of Biomedical Materials Research. 1998;41:474–480
  6. Geurtsen W. Biocompatibility of resin-modified filling materials. Critical Review in Oral Biology and Medicine. 2000;11(3):333–355
  7. Chang HH, Guo MK, Kasten FH, Chang MC, Huang GF, Wang YL, et al. Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials. 2005;26:745–753
  8. Engelmann J, Leyhausen G, Leibfritz D, Geurtsen W. Effect of TEGDMA on the intracellular glutathione concentration of human gingival fibroblasts. Journal of Biomedical Materials Research. 2002;63:746–751
  9. Stanislawski L, Lefeuvre M, Bourd K, Soheili-Majd E, Goldberg M, Perianin A. TEGDMA-induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species. Journal of Biomedical Materials Research Part A. 2003;66:476–482
  10. Lefeuvre M, Amjaad W, Goldberg M, Stanislawski L. TEGDMA induces mitochondrial damage and oxidative stress in human gingival fibroblasts. Biomaterials. 2005;2:5130–5137
  11. Brand KA, Hermifisse U. Aerobic glycolysis by proliferating cells: a protective strategy against reactive oxygen species. FASEB Journal. 1997;11(5):388–395
  12. Kletzien RF, Harris PK, Foellmi LA. Glucose-6-phosphate dehydrogenase: a “housekeeping” enzyme subject to tissue-specific regulation by hormones, nutrients, and oxidant stress. FASEB Journal. 1994;8:174–181
  13. Zimmer HG. Regulation of and intervention into the oxidative pentose phosphate pathway and adenine nucleotide metabolism in the heart. Molecular and Cellular Biochemistry. 1996;160–161:101–119
  14. Geurtsen W, Leyhausen G. Chemical–biological interactions of the resin monomer triethyleneglycol-dimethacrylate (TEGDMA). Journal of Dental Research. 2000;80:2046–2050
  15. Santangelo F. Intracellular thiol concentration modulating inflammatory response: influence on the regulation of cell functions through cysteine prodrug approach. Current Medicinal Chemistry. 2003;10:2599–2610
  16. Schmalz G. Use of cell cultures for toxicity testing of dental materials: advantages and limitation. Journal of Dentistry. 1994;22(Suppl. 2):S6–S11
  17. Scatena R, Bottoni P, Vincenzoni F, Messana I, Martorana GE, Nocca G, et al. Bezafibrate induces a mitochondrial derangement in human cell lines: a PPAR-independent mechanism for a peroxisome proliferator. Chemical Research in Toxicology. 2003;16:1440–1447
  18. Terasaka H, Kadoma Y, Sakagami H, Fujisawa S. Cytotoxicity and apoptosis-inducing activity of bisphenol A and hydroquinone in HL-60 cells. Anticancer Research. 2005;25:2241–2248
  19. Scatena R, Nocca G, De Sole P, Rumi C, Puggioni P, Remiddi F, et al. Bezafibrate as differentiating factor of human myeloid leukemia cells. Cell Death and Differentiation. 1999;6(8):781–787
  20. De Baeselier P, Schram E. Luminescent bioassay based on macrophage cell lines. Methods in Enzymology. 1986;133:507–530
  21. Li N, Ragheb K, Lawler G, Sturgis J, Rajwa B, Melendez JA, et al. Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production. Journal of Biological Chemistry. 2003;278:8516–8525
  22. Ellman G, Lysco H. A precise method for the determination of whole blood and plasma sulfhydryl groups. Analytical Biochemistry. 1979;93:98–102
  23. Wataha JC, Lewis JB, Lockwood PE, Rakich DR. Effect of dental metal ions on glutathione levels in THP-1 human monocytes. Journal of Oral Rehabilitation. 2000;27:508–516
  24. Zhang JW, Wang JY, Chen SJ, Chen Z. Mechanisms of all-trans retinoic acid-induced differentiation of acute promyelocytic leukemia cells. Journal of Bioscience. 2000;25:275–284
  25. Forman HJ, Fukuto JM, Torres M. Redox signaling: thiol chemistry defines which reactive oxygen and nitrogen species can act as second messengers. American Journal of Physiology. Cell Physiology. 2004;287:C246–C256
  26. Santos NC, Figueira-Coelho J, Martins-Silva J, Saldanha C. Multidisciplinary utilization of dimethyl sulfoxide: pharmacological, cellular, and molecular aspects. Biochemical Pharmacology. 2003;65:1035–1041
  27. Spagnuolo G, D’Antò V, Cosentino C, Schmalz G, Schweikl H, Rengo S. Effect of N-acetyl-l-cysteine on ROS production and cell death caused by HEMA in human primary gingival fibroblasts. Biomaterials. 2006;27:1803–1809
  28. Hagen T, D’Amico G, Quintero M, et al. Inhibition of mitochondrial respiration by the anticancer agent 2-methoxyestradiol. Biochemical and Biophysical Research Communications. 2004;322:923–929
  29. Lenaz G. The mitochondrial production of reactive oxygen species: mechanisms and implications in human pathology. IUBMB Life. 2001;52:159–164
  30. St-Pierre J, Buckingham JA, Roebuck SJ, Brand MD. Topology of superoxide production from different sites in the mitochondrial electron transport chain. Journal of Biological Chemistry. 2002;277:44784–44790
  31. Chen Q, Vazquez EJ, Moghaddas S, Hoppel CL, Lesnefsky EJ. Production of reactive oxygen species by mitochondria: central role of complex III. Journal of Biological Chemistry. 2003;278:36027–36031
  32. De Flora S, Izzotti A, D’Agostini F, Balansky RM. Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related end-points. Carcinogenesis. 2001;7:999–1013

PII: S0300-5712(06)00150-3

doi: 10.1016/j.jdent.2006.07.008

Journal of Dentistry
Volume 35, Issue 3 , Pages 187-194 , March 2007

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